產(chǎn)品簡介
?應(yīng)用范圍
制備的細胞核懸液可用于核轉(zhuǎn)錄組學(xué)（如 snRNA-seq/bulk RNA-seq），表觀遺傳學(xué)（如scATAC- seq/bulk ATAC- seq，CUT&Tag）等研究。
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鏡檢圖
應(yīng)用實例
伯優(yōu)?細胞核分離試劑盒已成功應(yīng)用于近20個物種的細胞核分離，涵蓋人、小鼠、大鼠等哺乳動物及扇貝、海葵等無脊椎動物。在組織適配性方面，本試劑盒已用于包括腦、肝臟、心臟、肺、腎、脾及各類腫瘤等200余種組織，累計20,000余例樣本的細胞核懸液制備。為單細胞核RNA測序提供了穩(wěn)定可靠的細胞核分離解決方案。
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單細胞核RNA測序?qū)崪y組織類型
English Translation
This product is specially designed to isolate high-purity single nuclei from animal tissues. Intact nuclei are released through tissue homogenization and cell membrane lysis, while the nuclear membrane stability and spatial structure of chromatin are well preserved. The optimized density gradient centrifugation effectively removes cell debris and contaminants, enabling the nuclei to meet strict quality requirements for cutting-edge research including single-cell omics and epigenetics.
Applications
The prepared nuclear suspension is applicable to nuclear transcriptomics (e.g., snRNA-seq, bulk RNA-seq) and epigenetics research (e.g., scATAC-seq, bulk ATAC-seq, CUT&Tag).
Microscopy Images
Application Cases
Boyou? Nucleus Isolation Kit has been successfully used for nucleus extraction from nearly 20 species, including mammals such as humans, mice and rats, as well as invertebrates like scallops and sea anemones. It is compatible with over 200 tissue types including brain, liver, heart, lung, kidney, spleen and various tumor tissues, with more than 20,000 sample preparations completed so far. It provides a stable and reliable solution for single-nucleus RNA sequencing.
Tested Tissue Types for Single-Nucleus RNA Sequencing
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